5OFT
Structural basis for OXA-48 dimerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 120.151, 120.151, 160.107 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.299 - 3.200 |
| R-factor | 0.1855 |
| Rwork | 0.182 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dtk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.675 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.185 | |
| Number of reflections | 11089 | |
| <I/σ(I)> | 8.36 | 2.45 |
| Completeness [%] | 93.7 | 96.33 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 14-18% PEG MME 5K and 0.1 M Bis Tris Propane pH 9-9.5. 2 uL hanging drops with a 1:1 ratio between protein and reservoir solution were equilibrated over 1 mL of reservoir solution |
