5OFN
Crystal structure of the heterotrimeric PriSLX primase from S. solfataricus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.052, 36.348, 110.941 |
| Unit cell angles | 90.00, 96.91, 90.00 |
Refinement procedure
| Resolution | 46.170 - 3.005 |
| R-factor | 0.2439 |
| Rwork | 0.241 |
| R-free | 0.29790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zt2 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.374 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | 3.113 |
| High resolution limit [Å] | 3.005 | 3.005 |
| Rmerge | 0.117 | 0.809 |
| Rpim | 0.077 | 0.520 |
| Number of reflections | 16582 | 1556 |
| <I/σ(I)> | 9.18 | 1.86 |
| Completeness [%] | 98.6 | 95.17 |
| Redundancy | 3.2 | 3.3 |
| CC(1/2) | 0.990 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% PEG 3350, 200 mM CaCl2, 0.5% n-octyl-beta-D-glucoside |
