5OEZ
Crystal structure of Leishmania major fructose-1,6-bisphosphatase in apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-13 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.260, 162.560, 170.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.770 - 2.410 |
| R-factor | 0.18886 |
| Rwork | 0.187 |
| R-free | 0.22536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fbp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.495 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.770 | 2.496 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.094 | |
| Number of reflections | 59755 | |
| <I/σ(I)> | 9.2 | 2.9 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 290.15 | 20% w/v PEG 3,350, 0.05 M citric acid, 0.05 M Bis-Tris propane |
