5OEY
Crystal structure of Leishmania major fructose-1,6-bisphosphatase in holo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.010, 104.150, 137.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.140 - 2.800 |
| R-factor | 0.19549 |
| Rwork | 0.193 |
| R-free | 0.24125 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fbp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.650 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.140 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.054 | 0.740 |
| Number of reflections | 33215 | 3280 |
| <I/σ(I)> | 12.3 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290.15 | 20% w/v PEG 3,350,0.05 M citric acid, 0.05 M Bis-Tris propane |
