5OEM

Crystal Structure of Interferon Regulatory Factor 9 IAD Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-08
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 32 2 1
Unit cell lengths	76.770, 76.770, 85.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.989 - 1.900
R-factor	0.2086
Rwork	0.207
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dsh
RMSD bond length	0.014
RMSD bond angle	1.263
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.989	35.989	2.010
High resolution limit [Å]	1.900	5.640	1.900
Rmerge	0.034	0.028	0.656
Rmeas	0.041	0.034	0.815
Total number of observations	66645
Number of reflections	22803	961	3314
<I/σ(I)>	14.81	38.7	1.45
Completeness [%]	96.7	96.6	88.3
Redundancy	2.923	2.688	2.396
CC(1/2)	0.999	0.998	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M HEPES pH 6.8, 1.5 M Ammonium phosphate monobasic and 0.1 M Ammonium sulphate