Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 63.500, 154.710, 77.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.580 - 3.150 |
| R-factor | 0.1852 |
| Rwork | 0.181 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lw1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.285 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.580 | 19.580 | 3.200 |
| High resolution limit [Å] | 3.150 | 12.000 | 3.150 |
| Rmerge | 0.086 | 0.020 | 0.265 |
| Rmeas | 0.102 | 0.024 | 0.312 |
| Number of reflections | 6825 | 115 | 306 |
| <I/σ(I)> | 16.05 | 47.45 | 5.32 |
| Completeness [%] | 99.3 | 73.7 | 100 |
| Redundancy | 3.584 | 3.017 | 3.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277.15 | 0.1 M Tris/HCl, 10% PEG 8000, 500 mM MgCl2, pH 7 |
