5OEC

Human Rab32 (18-201):GDP in complex with Salmonella GtgE (21-214) C45A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.822, 66.993, 110.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.954 - 2.300
R-factor	0.2116
Rwork	0.208
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cym 4mi7
RMSD bond length	0.002
RMSD bond angle	0.435
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.082	0.523
Number of reflections	16142
<I/σ(I)>	13.8	3.6
Completeness [%]	97.4	93
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	100 mM Bis-Tris, 25% PEG 3350