5OE7
Structure of OTULIN bound to the Met1-linked diubiquitin activity probe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2016-05-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.143, 101.143, 277.916 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.430 - 2.950 |
| R-factor | 0.227 |
| Rwork | 0.223 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3znz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.157 |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.430 | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.063 | 0.769 |
| Number of reflections | 11908 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 1.9-2.1 M Ammonium sulphate, 100 mM bis-tris |
