5ODO
Crystal Structure of the Oleate hydratase of Rhodococcus erythropolis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98141 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 261.747, 261.747, 127.304 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.466 - 2.640 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ia5 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.842 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.640 |
Number of reflections | 75230 |
<I/σ(I)> | 13.66 |
Completeness [%] | 99.8 |
Redundancy | 22.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 100 mM Bis-Tris/HCl 300 mM Magnesium formate 5% (v/v) glycerol |