5ODB

Crystal structure of the RA-associated mAb D10 (chimeric Fab fragment)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07201
Spacegroup name	P 1 21 1
Unit cell lengths	47.368, 66.714, 72.764
Unit cell angles	90.00, 93.85, 90.00

Refinement procedure

Resolution	47.260 - 1.530
R-factor	0.18885
Rwork	0.188
R-free	0.21387
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zuq 2dag
RMSD bond length	0.010
RMSD bond angle	1.471
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.260	1.560
High resolution limit [Å]	1.530	1.530
Rmerge	0.038	0.389
Rpim	0.037	0.367
Number of reflections	67539	3360
<I/σ(I)>	13.9	2.3
Completeness [%]	99.1	99.7
Redundancy	2.8	2.8
CC(1/2)	0.995	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.75	293	2.55 M ammonium sulfate, 0.1 M citrate pH 4.75, 0.1 M NDSB-256