5OD4

Avr2 effector protein from the fungal plant pathogen Fusarium oxysporum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9168
Spacegroup name	P 21 21 21
Unit cell lengths	33.410, 51.730, 74.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.100
R-factor	0.14026
Rwork	0.139
R-free	0.16899
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.823
Data reduction software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.540
High resolution limit [Å]	1.100
Number of reflections	53312
<I/σ(I)>	26.1
Completeness [%]	99.8
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES, pH 6.5; 25% (w/v) PEG 8K