5OCN

Crystal structure of the forkhead domain of human FOXN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	42.141, 78.203, 263.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.913 - 2.700
R-factor	0.2527
Rwork	0.251
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c6y
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.920	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.101	1.321
Rmeas	0.044
Rpim		0.713
Number of reflections	24950	3240
<I/σ(I)>	11.8	1.5
Completeness [%]	99.8	99.9
Redundancy	7.1	7.3
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	32 % PEG 3350, 0.25 M Potassium Citrate tribasic, 10% Ethylene Glycol