5OCL

Nanobody-anti-VGLUT nanobody complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 4 21 2
Unit cell lengths	126.295, 126.295, 240.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.812 - 2.200
R-factor	0.1889
Rwork	0.188
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4WGV
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.166	1.490
Number of reflections	99098	12196
<I/σ(I)>	14.7	2.4
Completeness [%]	100.0	100
Redundancy	26.2	26.6
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	3.5 M Sodiumformate