5OBH

Crystal structure of glycine binding protein in complex with bicuculline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	150
Detector technology	CCD
Collection date	2016-11-16
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54157
Spacegroup name	P 21 21 21
Unit cell lengths	71.243, 132.236, 132.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.820 - 2.400
R-factor	0.23054
Rwork	0.230
R-free	0.24944
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oan
RMSD bond length	0.009
RMSD bond angle	1.418
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.820	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.130	0.776
Number of reflections	49817	4510
<I/σ(I)>	7.9	1.8
Completeness [%]	100.0	100
Redundancy	6.2	6.2
CC(1/2)	0.995	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Reservoir buffer: 0.2 M ammonium formate, 20 % PEG 3350 Protein buffer: 50 mM tris, 250 mM NaCl, pH 7.5. 2 mM bicuculline