5OAS

Crystal structure of malate synthase G from Pseudomonas aeruginosa in apo form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	71.171, 81.197, 137.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.926 - 1.620
R-factor	0.1683
Rwork	0.167
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n8i
RMSD bond length	0.008
RMSD bond angle	0.897
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	69.962
High resolution limit [Å]	1.620
Number of reflections	95973
<I/σ(I)>	11.75
Completeness [%]	94.2
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292.15	PEG 4000 ammonium sulfate glycerol