5O9W
Thebaine 6-O-demethylase (T6ODM) from Papaver somniferum in complex with 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2016-12-21 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.044, 87.424, 94.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.430 - 1.850 |
| R-factor | 0.16428 |
| Rwork | 0.162 |
| R-free | 0.21358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o7y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.414 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.430 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.355 |
| Number of reflections | 33795 | |
| <I/σ(I)> | 15.773 | 2.271 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.4 | 277 | 19% PEG 3350, 200 mM disodium 2-oxoglutarate. Concentration of protein 37.5 mg/ml. |
