5O9U
HsNMT1 in complex with CoA and Myristoylated-GCSVSKKK octapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.236, 79.779, 178.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.037 - 1.850 |
| R-factor | 0.1573 |
| Rwork | 0.155 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c2z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.046 | 0.171 |
| Number of reflections | 71441 | 6884 |
| <I/σ(I)> | 15.1 | 5.5 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 5.4 | 4.9 |
| CC(1/2) | 0.998 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21% PEG 6000, 0.1M sodium citrate pH 5.6, 0.1M magnesium chloride, 0.1M sodium chloride |
