5O8P
The crystal structure of DfoA bound to FAD, the desferrioxamine biosynthetic pathway cadaverine monooxygenase from the fire blight disease pathogen Erwinia amylovora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 164.090, 164.090, 168.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.570 - 2.750 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.22398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tlx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.570 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmeas | 0.066 | 0.670 |
| Number of reflections | 60324 | 4397 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES, 1.45 M ammonium sulphate |
