5O8M

Crystal structure of the MmI1 YTH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97242
Spacegroup name	P 1 21 1
Unit cell lengths	57.020, 58.330, 94.090
Unit cell angles	90.00, 106.59, 90.00

Refinement procedure

Resolution	90.170 - 1.450
R-factor	0.15775
Rwork	0.157
R-free	0.17916
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.197
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.200	1.510
High resolution limit [Å]	1.450	1.450
Rmerge	0.088	0.580
Number of reflections	102990	11613
<I/σ(I)>	10.68	2.63
Completeness [%]	98.1	96.9
Redundancy	4.7	4.7
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M ammonium sulfate, 0.1 M Tris pH 8.5, 25 % PEG3350.