5O8M
Crystal structure of the MmI1 YTH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.020, 58.330, 94.090 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 90.170 - 1.450 |
| R-factor | 0.15775 |
| Rwork | 0.157 |
| R-free | 0.17916 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.197 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.200 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.088 | 0.580 |
| Number of reflections | 102990 | 11613 |
| <I/σ(I)> | 10.68 | 2.63 |
| Completeness [%] | 98.1 | 96.9 |
| Redundancy | 4.7 | 4.7 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M ammonium sulfate, 0.1 M Tris pH 8.5, 25 % PEG3350. |
