5O8I

Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) crystallized at P212121 space group, and refined to 1.27 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 21 21 21
Unit cell lengths	46.031, 76.364, 80.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.430 - 1.270
R-factor	0.10183
Rwork	0.100
R-free	0.13326
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw2
RMSD bond length	0.030
RMSD bond angle	2.652
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	MOLREP (11.4.06)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.430	1.290
High resolution limit [Å]	1.270	1.270
Rmerge	0.044	0.535
Rpim	0.038	0.461
Number of reflections	74711	3586
<I/σ(I)>	14.1
Completeness [%]	98.6	95.8
Redundancy	4.1	3.8
CC(1/2)	0.999	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	281	20% w/v PEG 3350, 0.1 M Bis-Tris Propane pH 8.5, 0.2 M Sodium/Potassium Phosphate