5O7Y
Thebaine 6-O-demethylase (T6ODM) from Papaver somniferum in complex with succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2016-10-18 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.231, 87.028, 94.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.510 - 1.970 |
| R-factor | 0.16485 |
| Rwork | 0.163 |
| R-free | 0.20674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xae |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.510 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.136 | 0.653 |
| Number of reflections | 28189 | |
| <I/σ(I)> | 17.71 | 2.271 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 277 | 125 mM sodium succinate, 22% PEG 3350. Concentration of protein 37.5 mg/ml. |
