5O78

Crystal structure of Omp35 from Enterobacter aerogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	111.570, 115.940, 216.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	108.330 - 2.850
R-factor	0.21597
Rwork	0.214
R-free	0.26034
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1omf
RMSD bond length	0.010
RMSD bond angle	1.462
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.330	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.110
Number of reflections	27313
<I/σ(I)>	6.3
Completeness [%]	85.0
Redundancy	2.5
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M Lithium Chloride, 0.05 M magnesium sulfate, 8% PEG 8000