5O77
Klebsiella pneumoniae OmpK35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 63 |
| Unit cell lengths | 77.312, 77.312, 114.044 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.410 - 1.500 |
| R-factor | 0.15763 |
| Rwork | 0.157 |
| R-free | 0.18476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gcp |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.087 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.410 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rpim | 0.044 | 5.210 |
| Number of reflections | 61689 | |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 13% PEG 2000MME, 0.1 M calcium chloride, 0.1 M Tris |
