5O6R
Structure of beta-phosphoglucomutase D10N mutant in complex with glucose-1-phosphate and aluminium tetrafluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.300, 54.900, 107.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.360 |
| R-factor | 0.11652 |
| Rwork | 0.115 |
| R-free | 0.14898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o08 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.741 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.780 | 1.440 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.027 | 0.110 |
| Rpim | 0.077 | |
| Number of reflections | 43148 | 16940 |
| <I/σ(I)> | 24.5 | 7.4 |
| Completeness [%] | 93.6 | 91.3 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 19-21 % PEG 3350 and 50 mM Mg acetate |
