5O6I
Structures and dynamics of mesophilic variants from the homing endonuclease I-DmoI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.000, 70.480, 107.320 |
| Unit cell angles | 90.00, 119.81, 90.00 |
Refinement procedure
| Resolution | 56.137 - 2.250 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.140 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 64736 |
| <I/σ(I)> | 11.7 |
| Completeness [%] | 98.0 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5-6 % PEG4000, 0.07 M NaAc pH = 4.6-5.5 and 30% Glycerol |
