5O6G
Structures and dynamics of mesophilic variants from the homing endonuclease I-DmoI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.280, 70.500, 107.150 |
| Unit cell angles | 90.00, 119.89, 90.00 |
Refinement procedure
| Resolution | 56.184 - 2.750 |
| R-factor | 0.1803 |
| Rwork | 0.177 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 93.100 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 35510 |
| <I/σ(I)> | 9.4 |
| Completeness [%] | 97.5 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5-6 % PEG4000, 0.07 M NaAc pH = 4.6-5.5 and 30% Glycerol |
