5O6D
Structure of ScPif1 in complex with polydT and ATPgS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977760 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.406, 90.783, 186.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.000 - 3.283 |
| R-factor | 0.2553 |
| Rwork | 0.252 |
| R-free | 0.32190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o6b |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.697 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 3.400 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.160 | 0.710 |
| Number of reflections | 17964 | 1952 |
| <I/σ(I)> | 7.62 | 2.27 |
| Completeness [%] | 89.7 | 99.54 |
| Redundancy | 6.5 | 6.2 |
| CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 100mM NaAc-HAc pH5.5 240mM NaF 14%-16% PEG8000 |
