5O6C
Crystal Structure of a threonine-selective RCR E3 ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2823 |
| Spacegroup name | P 61 |
| Unit cell lengths | 82.578, 82.578, 103.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.750 |
| R-factor | 0.17245 |
| Rwork | 0.172 |
| R-free | 0.19570 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.962 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.051 | 0.404 |
| Number of reflections | 40007 | |
| <I/σ(I)> | 18.3 | 2.4 |
| Completeness [%] | 99.3 | 93.3 |
| Redundancy | 6.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 23.3 mM Na2HPO4 23.3 mM (NH4)2SO4 23.3 mM NaNO3 18 % PEG500 MME 9 % PEG20000 |
