5O67
Crystal structure of the FapF polypeptide transporter - F103A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.753, 125.816, 81.908 |
| Unit cell angles | 90.00, 96.68, 90.00 |
Refinement procedure
| Resolution | 81.350 - 2.840 |
| R-factor | 0.23482 |
| Rwork | 0.232 |
| R-free | 0.29527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o65 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.727 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.350 | 2.890 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rpim | 0.149 | |
| Number of reflections | 33293 | |
| <I/σ(I)> | 7.2 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0/05 - 0.1 M sodium citrate, 0.1 M sodium chloride, 20-30 % PEG400 |
