5O65
Crystal Structure of the Pseudomonas functional amyloid secretion protein FapF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.260, 124.731, 80.359 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 2.640 - 2.500 |
| R-factor | 0.22343 |
| Rwork | 0.222 |
| R-free | 0.25928 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.736 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.380 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.059 | 0.400 |
| Number of reflections | 48100 | |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 - 0.1 M sodium citrate, 0.1 M sodium chloride, 20-30 % PEG 400 |
