5O5L
X-ray structure of a bacterial adenylyl cyclase soluble domain, solved at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2015-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.89 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.480, 84.120, 310.750 |
| Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
| Resolution | 49.380 - 2.700 |
| R-factor | 0.267 |
| Rwork | 0.265 |
| R-free | 0.28900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.380 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 98383 |
| <I/σ(I)> | 22.3 |
| Completeness [%] | 98.5 |
| Redundancy | 44.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M NaCacodylate pH 6.5, 0.2 M LiSO4, 8-10% PEG 1000, 8-10% PEG 8000 |
