5O51
AfRom2 CNH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.980, 93.710, 52.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.870 - 2.010 |
| R-factor | 0.20692 |
| Rwork | 0.205 |
| R-free | 0.24824 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.917 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.870 |
| High resolution limit [Å] | 2.010 |
| Rmerge | 0.095 |
| Number of reflections | 26362 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 99.8 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.02 M L-Na-Glutamate; 0.02 M Alanine (racemic);0.02M Glycine; 0.02M Lysine HCl (racemic); 0.02M Serine (racemic);0.1 M tris Bicine buffer (pH 8.5); 20% v/v Ethylene glycol; 10 % w/v PEG 8000 |
