5O4T

Crystal structure of the human BRPF1 bromodomain in complex with BZ061

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	60.639, 60.639, 63.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.460 - 1.500
R-factor	0.1853
Rwork	0.182
R-free	0.21200
Starting model (for MR)	4lc2
RMSD bond length	0.005
RMSD bond angle	0.723
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.460	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.090	0.642
Number of reflections	21891	1029
<I/σ(I)>	16.9
Completeness [%]	99.4	97.4
Redundancy	13.2	12.9
CC(1/2)	0.997	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium nitrate, 20% PEG3350