5O47
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructosyl-hydroxytyrosol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.616, 205.356, 146.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.980 - 1.910 |
| R-factor | 0.16854 |
| Rwork | 0.168 |
| R-free | 0.18213 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ann |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.980 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.072 | |
| Rpim | 0.030 | |
| Number of reflections | 175297 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Protein was crystallized from 1.3M Sodium Citrate tribasic dihydrate |
