5O3H

Human Brd2(BD2) mutant in complex with 9-ME-Am1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-11
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	52.143, 71.837, 31.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.483 - 1.400
R-factor	0.1485
Rwork	0.148
R-free	0.16360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qeu
RMSD bond length	0.011
RMSD bond angle	1.154
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.483	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.062	0.102
Number of reflections	24057	2228
<I/σ(I)>	21.35
Completeness [%]	98.7	93.14
Redundancy	7.5	3.7
CC(1/2)	0.999	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris pH 9.0 45% Pentaerythritol propoxylate (5/4 PO/OH)