5O20
Structure of Nrd1 RNA binding domain in complex with RNA (UUAGUAAUCC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979260 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.157, 66.157, 156.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.960 - 3.530 |
| R-factor | 0.2273 |
| Rwork | 0.225 |
| R-free | 0.26861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.960 | 3.870 |
| High resolution limit [Å] | 3.530 | 3.530 |
| Rmerge | 0.081 | 0.435 |
| Number of reflections | 4716 | 1077 |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 9.7 | 9.7 |
| CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 1M Sodium Potassium Phosphate pH 7.4 |
