5O1Y
Structure of Nrd1 RNA binding domain in complex with RNA (GUAA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.936, 64.936, 158.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.080 - 2.450 |
| R-factor | 0.1977 |
| Rwork | 0.197 |
| R-free | 0.21724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o1w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.080 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.040 | 0.630 |
| Rpim | 0.100 | 0.154 |
| Number of reflections | 12717 | |
| <I/σ(I)> | 28.2 | 3.4 |
| Completeness [%] | 96.1 | |
| Redundancy | 16.7 | 17.7 |
| CC(1/2) | 1.000 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 1M Sodium Potassium Phosphate pH 7.4 |
