5O1X
Structure of Nrd1 RNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979300 |
| Spacegroup name | P 65 |
| Unit cell lengths | 58.390, 58.390, 92.446 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.570 - 1.600 |
| R-factor | 0.17904 |
| Rwork | 0.178 |
| R-free | 0.20805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o1w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.450 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.081 | 0.600 |
| Rpim | 0.018 | 0.136 |
| Number of reflections | 23596 | |
| <I/σ(I)> | 26.82 | 6.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 | |
| CC(1/2) | 0.990 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M Potassium Thiocyanate, 23% PEG 3350 |
