5O1V
Crystal structure of WNK3 kinase domain in a monophosphorylated apo state (A-loop swapped)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.966, 67.484, 79.895 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 51.486 - 1.723 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.686 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.480 |
| High resolution limit [Å] | 1.720 |
| Number of reflections | 56586 |
| <I/σ(I)> | 18.1 |
| Completeness [%] | 85.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% PEG4000, 10% 2-propanol, 0.1M HEPES pH 7.5. 2:1 protein to reservoir. |
