5O1J
Lytic transglycosylase in action
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.135, 69.122, 143.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.660 - 1.900 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.24906 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4YIM |
RMSD bond length | 0.016 |
RMSD bond angle | 1.592 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 71.660 |
High resolution limit [Å] | 1.900 |
Number of reflections | 54011 |
<I/σ(I)> | 7.81 |
Completeness [%] | 99.4 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 0.2M Zinc acetate, or 0.1 M Sodium cacodylate pH 6.5, 0.1M 18%PEG 8000 |