5O10
Y48H mutant of human cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.937, 36.498, 60.700 |
| Unit cell angles | 90.00, 116.65, 90.00 |
Refinement procedure
| Resolution | 54.250 - 1.360 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zcf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.179 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.250 | 1.390 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.072 | 1.115 |
| Rpim | 0.031 | 0.510 |
| Number of reflections | 45812 | 2124 |
| <I/σ(I)> | 13.3 | 1.4 |
| Completeness [%] | 95.3 | 90.2 |
| Redundancy | 6.2 | 5.4 |
| CC(1/2) | 0.998 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 33% PEG 6000 100mM Tris pH 7.5 |
