5O0Y
TLK2 kinase domain from human
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 126.020, 126.020, 126.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.110 - 2.860 |
| R-factor | 0.18392 |
| Rwork | 0.183 |
| R-free | 0.20979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.760 | 2.959 |
| High resolution limit [Å] | 2.850 | 2.857 |
| Number of reflections | 15761 | 15761 |
| <I/σ(I)> | 13.07 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 mM HEPES pH 7, 2M Li2SO4, 10mM MgCl2 1M Na/K tartrate, 0.1M Tris pH7, 200mM LiSO4 |
