5O0U
Crystal structure of tarantula venom peptide Protoxin-II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-07-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 37.669, 37.669, 103.944 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.650 - 0.990 |
| R-factor | 0.12668 |
| Rwork | 0.126 |
| R-free | 0.13985 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.189 |
| Phasing software | SHARP (2.8.5) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 1.010 |
| High resolution limit [Å] | 0.990 | 0.993 |
| Rmerge | 0.062 | 0.496 |
| Rpim | 0.020 | 0.361 |
| Number of reflections | 14967 | 341 |
| <I/σ(I)> | 25 | |
| Completeness [%] | 92.9 | 43.3 |
| Redundancy | 8.7 | 3.4 |
| CC(1/2) | 0.998 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2M lithium sulfate, 100 mM Tris-HCl (pH 8), 2% v/v PEG 400 |
