5O0P
Deglycosylated Nogo Receptor with native disulfide structure 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.208, 46.891, 121.795 |
| Unit cell angles | 90.00, 123.65, 90.00 |
Refinement procedure
| Resolution | 43.525 - 2.000 |
| R-factor | 0.1856 |
| Rwork | 0.184 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.211 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.080 | 1.970 |
| High resolution limit [Å] | 2.000 | 1.920 |
| Rmeas | 0.109 | 1.329 |
| Number of reflections | 48789 | |
| <I/σ(I)> | 8.5 | 1 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.998 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.05 M citric acid, 0.05 M Bis-Tris propane pH 5.0, 16 % (w/v) PEG3350 |
