5O0L

Deglycosylated Nogo Receptor with native disulfide structure 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	72.909, 38.597, 119.610
Unit cell angles	90.00, 106.20, 90.00

Refinement procedure

Resolution	38.404 - 2.511
R-factor	0.1996
Rwork	0.197
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ozn
RMSD bond length	0.002
RMSD bond angle	0.521
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	2.620
High resolution limit [Å]	2.510	2.510
Number of reflections	20826
<I/σ(I)>	12.2	3.3
Completeness [%]	94.6	89.6
Redundancy	2.8	2.6
CC(1/2)	0.992	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	Result of a co-crystallization trial with the Endo-Hf-deglycosylated extracellular domain of mouse MAG (UNIPROT P20917, residues 20-508) in SEC buffer, mixed at equimolar stoichiometry. NgRa was also Endo-Hf-deglycosylated. Crystals were grown in a condition of 0.5 M LiCl, 0.05 M citric acid pH 4.0, 15 % (w/v) PEG6000.