5O0K
Deglycosylated Nogo Receptor with native disulfide structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.624, 46.586, 120.670 |
| Unit cell angles | 90.00, 123.45, 90.00 |
Refinement procedure
| Resolution | 43.783 - 2.300 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.650 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.783 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 31309 | |
| <I/σ(I)> | 5.9 | 1.5 |
| Completeness [%] | 98.7 | 98.6 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.936 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.05 M citric acid pH 5.0, 15 % (w/v) polyethylene glycol (PEG) 6000 |
