5NYI
Crystal Structure of Hsp90-alpha N-Domain in complex with Resorcinol derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.430, 89.088, 99.944 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.080 - 1.440 |
| R-factor | 0.1819 |
| Rwork | 0.181 |
| R-free | 0.19970 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHATHR |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.063 | 1.591 |
| Number of reflections | 53759 | |
| <I/σ(I)> | 16.97 | 1.11 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 6.56 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 100 mM Bis-Tris buffer pH 8.5, 20% PEG 3,350, 200 mM NaF |
