5NXS

Crystal Structure of Human Pro-myostatin Precursor at 4.2 A Resolution with Experimental Phases from SeMet labelling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9797
Spacegroup name	I 2 3
Unit cell lengths	196.830, 196.830, 196.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.410 - 4.190
R-factor	0.276
Rwork	0.274
R-free	0.30100
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.530
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.414	98.414	4.267
High resolution limit [Å]	4.194	11.380	4.194
Rmeas	0.148	0.099	2.732
Rpim	0.012	0.008	0.238
Total number of observations	1517107	71017	60405
Number of reflections	9470
<I/σ(I)>	28.6	80.1	3
Completeness [%]	100.0	99.8	100
Redundancy	160.2	138.2	128
CC(1/2)	0.996	0.996	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.2	292	10 mg/mL protein, reservoir: 0.1M NaAcetate, 1M (NH4)3PO4: cryo-protection: 26% ethylene glycol