5NXF
Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, residues 795 to 1289, at 1.9 Angstrom.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.107, 75.943, 149.128 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 46.120 - 1.900 |
| R-factor | 0.17151 |
| Rwork | 0.171 |
| R-free | 0.20785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uxf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.115 | 0.425 |
| Number of reflections | 161501 | 23029 |
| <I/σ(I)> | 10 | 3.2 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | between 4 and 7 % (w/v) PEG 6000 100 mM TrisHCl |
