5NXB

Mouse galactocerebrosidase in complex with saposin A

Replaces:  5N8K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92818
Spacegroup name	P 62 2 2
Unit cell lengths	187.183, 187.183, 360.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	162.110 - 3.600
R-factor	0.218
Rwork	0.217
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cce 4ddj
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	180.120	3.700
High resolution limit [Å]	3.600	3.600
Rmerge	0.386	2.946
Number of reflections	44024	3316
<I/σ(I)>	9.3
Completeness [%]	100.0	100
Redundancy	25.6	25.2
CC(1/2)	0.945	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	800 nL of protein (90 uM GALC plus 180 uM SapA) was mixed with 800 nL of reservoir solution (75 mM Sodium citrate pH 5.6, 11% PEG 3350) and equilibrated against 200 uL reservoirs at 20C.